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DHA Phase 5, Lahore DHA Phase 5
Laboratory/Microbiology

Microbiology bench

CareWell Diagnostics · Gulberg III processing lab · Microbiologist Dr. Faraz Ahmed · Breakpoints CLSI M100-Ed36 (2026)

Cultures in progress
64
Urine 38 · Blood 11 · Wound 9 · HVS 6
Positive blood cultures
3
Gram stain phoned to doctor ≤ 1 h
MDR alerts (7 days)
9
ESBL 6 · MRSA 2 · CRE 1
Urine C/S TAT
46 h
Target 48 h · 92% within target

Culture worklist by stage

Synced with VITEK 2 & BacT/ALERT via MedX Bridge
Day 0 · Inoculated 21
M26-08841Urine
Nadia Perveen · 34 F · MSU
Blood agarCLED
Inoculated 09:12 · read at 09:00 tomorrow
M26-08847Wound
Rizwan Haider · 58 M · diabetic foot
BAMacConkeyChocolate
Gram: Gram-positive cocci in clusters (prelim sent)
Day 1 · Growth read 18
M26-08790Urine
Zainab Bibi · 62 F · catheter
Growth >10⁵ CFU/mL · lactose fermenter
Sent to VITEK ID + AST card GN-AST N417
M26-08802HVS
Hira Khan · 28 F · antenatal
No pathogen isolated at 24 h
Final at 48 h
Day 2 · ID + AST 14
M26-08741ESBL
Abdul Rehman · 55 M · urine
E. coli >10⁵ CFU/mL · AST ready for review
Review AST
B26-00412Blood
Usman Ali · 41 M · fever 10 days
Salmonella Typhi · XDR screen pending
Final reported 11
M26-08690Final
Sadia Akhtar · urine
Mixed growth — likely contamination; repeat MSU advised
M26-08655Final
Kashif Mehmood · pus
S. aureus (MRSA) · infection-control notified

M26-08741 · Abdul Rehman · Urine (MSU)

Collected 11 Oct 08:40 · Johar Town centre · Panel: Engro Fertilizers · Ref: Dr. Usman Tariq
Preliminary sent · Final pending
ESBL-producing E. coli — resistance alert

Ceftriaxone R, cefotaxime R, ESBL phenotypic confirm positive. Report will carry the ESBL comment; infection-control notice goes to the referring doctor. Carbapenems unlocked by cascade rule CASC-ENT-03.

Gram stain
Gram-negative bacilli
Pus cells 25–30/HPF
Colony count
Organism (isolate 1 of 1)
Escherichia coli
VITEK 2 · 99% probability · WHONET code eco
AntibioticMethodValueResultAWaReTierOn report
NitrofurantoinDisk22 mmSAccess1Shown
FosfomycinDisk19 mmSWatch1Shown
Trimethoprim–sulfamethoxazoleMIC≥320RAccess1Shown
CiprofloxacinMIC≥4RWatch2Shown
CeftriaxoneMIC≥64RWatch2Shown
AmikacinMIC≤2SAccess2Shown
Piperacillin–tazobactamMIC8SWatch2Shown
MeropenemMIC≤0.25SWatch3Cascade-released
ColistinBMD≤0.5SReserve4Suppressed
Ceftazidime–avibactamDisk25 mmSReserve4Suppressed

Blood culture · time to positivity

BacT/ALERT 3D
B26-00412
18 h ⚑
B26-00418
25 h ⚑
B26-00420
Day 3 · NG
B26-00423
Day 1 · NG

⚑ Flagged positive → Gram stain → critical call within 1 h. Negative bottles are finalised "No growth after 5 days".

Critical call log

B26-00412 · GNB in blood
Informed Dr. Usman Tariq (Consultant) · 0300-xxxx221 · read-back ✓ · 10:42 by Dr. Faraz
Done
B26-00418 · GPC clusters
Attempt 1 no answer 11:05 · escalate at 12:05
Retry

Antibiogram · E. coli (urine, 90 days)

Nitrofurantoin
91%
Fosfomycin
88%
Amikacin
86%
Ceftriaxone
34%
Ciprofloxacin
29%

% susceptible, first isolate per patient (CLSI M39). n = 412.